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蛋白与药物互作专题八:MST和CETSA在分子互作中的应用
此外,蛋白在纯化过程中其构象可能发生改变,所获得的药物-靶标相互作用信息不能反映活细胞内的真实情况。而且很多蛋白很难重组表达纯化,进而限制了利用生物物理分子互作技术鉴定化合物和蛋白的相互作用。这时,也可以采用 细胞热迁移技术 (cell ...
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