编辑推荐： 本研究针对HIV-1潜伏库量化难题，创新性地将完整前病毒DNA检测技术 (IPDA)适配至芯片数字PCR (dPCR)平台，采用CCR5替代传统RPP30作为参考基因，系统评估了其在HIV-1不同亚型中的检测效能。结果表明该技术显著减少操作时间，在ART治疗者中实现高特异性检测，为非B亚型样本的精准量化提供 ...

Extremely early antiretroviral therapy during HIV-1 subtype C infection leads to faster decline in genome-intact viruses, reduced genetic complexity, and immune escape, and may enhance reservoir ...

海力特生物是位于广州市黄埔区的新型分子生物诊断高新技术企业，其自主研发的人类免疫缺陷病毒Ⅰ型 (HIV-1) DNA 检测试剂盒 (PCR-荧光探针法)，近日获得国家药品监督管理局 (NMPA)颁发的III类《医疗器械注册证》， 成为全世界范围内首款获批的DNA载量检测试剂。

据悉，此次获批的HIV-1 DNA载量检测试剂盒突破了传统PCR无法同时保证技术灵敏性和特异性的世界级行业技术瓶颈，以精准、稳定、便利、多场景应用等优势为艾滋病诊疗起到关键作用。 作为一家以功能性治愈为导向… ...

Single-Cell-in-Droplet (scd)PCR is a technique developed to enable intra-droplet cell lysis and PCR amplification of intracellular RNA without interfering with histone/chromatin bound DNA or PCR ...

BMC recently implemented a new HIV-1/HIV-2 Qualitative RNA PCR (HIV RNA QUAL) cleared by the US Food and Drug Administration to (1) replace antibody discrimination follow-up testing after a reactive ...

由HIV-1编码的整合酶介导的“整合”，是逆转录病毒感染的标志，整合的前病毒DNA是细胞中HIV-1 DNA最稳定的一种形式。证据表明，未整合的DNA可以具有转录活性，并在包括静息CD4 +T细胞在内的… ...

Rapid and Sensitive Qualitative Duoplex Real-Time PCR Method for Discriminatory and Confirmatory Diagnosis of HTLV-1 and HTLV-2 Infections: Brazilian Multicentric Study ...

Whole blood testing for total HIV-1 DNA is more convenient, economical, and operational than using peripheral blood mononuclear cell samples.

HIV proviral DNA changes from baseline were inversely related to CD4 + /CD8 + T-cell ratio and CD4 + T-cell changes, and directly related to the changes of CD8 + T cells. Further, HIV DNA decay ...

Reactivation of latent viral reservoirs is on the forefront of HIV-1 eradication research. However, it is unknown if latency reversing agents (LRAs) increase the level of viral transcription from ...