One part was submerged in Coomassie brilliant blue R-250 for protein bands visualization and the other part was renatured ... The plate was dried and dipped into the staining solution (30% ammonium ...
The phenol-sulfuric acid method, Coomassie brilliant blue method, and m-hydroxy biphenyl method were used to evaluate the content of the overall sugar, proteins, and uronic acid in the polysaccharide ...