Comparison of expression data requires normalization. The optimum normalization method depends on sample type, with the most common being to normalize to reference genes. It is critical to select ...

The MIQE guidelines and papers such as the one discussed here can be applied to avoid pitfalls. Reverse-transcription quantitative polymerase chain reaction (RT-qPCR) is everywhere. Its ubiquity, ...

Stephen Bustin knew something was wrong as soon as he visited the laboratory. He was investigating reports that using a technique called real-time quantitative PCR (qPCR) researchers had identified ...

This FDA-led Sequencing Quality Control (SEQC) study compared RNA-seq data generated using different NGS platforms to benchmark their performance. The authors compared different NGS platforms and how ...