We therefore developed a strategy to assemble the full–length HIV–1 clones by using PCR–mediated recombination (Fig. 1). The four DNA fragments (A, B, C and D) encompassing the full–length ...
Early steps of infection by HIV-1 involve entry of the viral core ... To generate a standard curve for Alu-PCR, we prepared cellular DNA containing proviruses integrated at many locations as ...
Use the growth medium, which includes PCR primers ... The naked-DNA vaccine is complete. Select another pathogen. Congratulations. You have just produced a naked-DNA HIV vaccine.