If you can't delete an ISO file as the file is open in your System, check if it is mounted as a drive or not. Uses these proven fixes to resolve the issue.

Why Cant I Delete, Edit, or Rename a File That Is in Use? Windows prevents you from deleting, moving, or renaming files that are in use to keep your computer system stable.

If you're trying to delete a Mac app but can't because it's still open, don't worry! There are a few ways to fix this issue.

If you receive File in Use. The action can't be completed because the file is open in another program even if file is closed in another program, see this.